Enzeli will face 12 rivals when he attempts to defend his Gold Cup crown at Royal Ascot on Thursday.
John Oxx's stayer will bid to continue the stable's fine run following the Derby success of Sinndar.
The Kahyasi colt returned to action at Leopardstown last month, finishing a short-head second to Royal Rebel, and will face stiff competition from Kayf Tara who is also be attempting to win his second Gold Cup.
Godolphin's six-year-old, who made a successful reappearance in the Yorkshire Cup, took the stayers' crown in 1998 but could manage only third place behind Enzeli 12 months ago.
John Dunlop has Celeric, Orchestra Stall and San Sebastian in the Gold Cup, which he won with Ragstone in 1974.
But the fear of firmish ground means mudlark Orchestra Stall is not certain to take his chance.
Dunlop's racing secretary Marcus Hosgood said: "We have declared Orchestra Stall but he will only run if there is significant rain."
Henry Cecil will attempt to win the two-and-a-half-mile Group One event with Endorsement, who will be having her first race of the season.
The Warren Place trainer's Le Moss won the Gold Cup in 1980 on his seasonal debut.
Life Is Life is the other filly in the race, having earned her place in the line-up by finishing a three-quarter-length second to Daliapour at Chester last time.
Rainbow High and Persian Punch, 10th and 12th respectively in last year's race, are set to go to post once again.
Other interesting contenders are Martin Pipe's Doncaster Cup winner Far Cry and the James Fanshawe-trained Arctic Owl.
Ashgar represents Clive Brittain and Gerard Butler saddles Compton Ace, who finished second at Newbury on his recent return from injury.
The only withdrawals at final declaration stage were Sea Wave and Hirapour.
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